2024

Microglia drive diurnal variation in susceptibility to inflammatory blood-brain barrier breakdown.
Lawrence JH, Patel A, King MW, Nadarajah CJ, Daneman R, Musiek ES.
JCI Insight. 2024 November 8. doi:10.1172/jci.insight.180081.

Microglia are not necessary for maintenance of blood-brain barrier properties in health, but PLX5622 alters brain endothelial cholesterol metabolism.
Profaci CP, Harvey SS, Bajc K, Zhang TZ, Jeffrey DA, Zhang AZ, Nemec KM, Davtyan H, O’Brien CA, McKinsey GL, Longworth A, McMullen TP, Capocchi JK, Gonzalez JG, Lawson DA, Arnold TD, Davalos D, Blurton-Jones M, Dabertrand F, Bennett FC, Daneman R.
Neuron. 2024 August 13. doi:https://doi.org/10.1016/j.neuron.2024.07.015.

Mild hypoxia accelerates cerebral cavernous malformation disease through CX3CR1-CX3CL1 signaling.
Frias-Anaya, E, Gallego-Gutierrez H, Gongol B, Weinsheimer S, Lai CC, Orecchioni M, Sriram A, Bui CM, Nelsen B, Hale P, Pham A, Shenkar R, DeBiasse D, Lightle R, Girard R, Li Y, Srinath A, Daneman R, Nudleman E, Sun H, Guma M, Debrac A, Mesarwi OA, Ley K, Kim H, Awad IA, Ginsberg MH, Lopez-Ramirez MA.
Atheroscler Thromb Vasc Biol. 2024 April 25. doi:10.1161/ATVBAHA.123.320367.

Notch3 directs differentiation of brain mural cells from human pluripotent stem cell-derived neural crest.
Gastfriend BD, Snyder ME, Holt HE, Daneman R, Palecek SP, Shusta EV.
Science Advances. 2024 February 2. doi:10.1126/sciadv.adi1737.

Dural mural cells paint an anti-inflammatory picture.
Lummis NC, Gastfriend BD, Daneman R.
J Exp Med. 2024 January 25. doi:10.1084/jem.20232263.

2023

CD98hc is a target for brain delivery of biotherapeutics.
Chew KS, Wells RC, Moshkforoush A, Chan D, Lechtenberg KJ, Train HL, Chow J, Kim DJ, Robles-Colmenares Y, Srivastava DB, Tong RK, Tong M, Xa K, Yang A, Zhou Y, Akkapeddi P, Annamalai L, Bajc K, Blanchette M, Cherf GM, Earr TK, Gill A, Huynh D, Joy D, Knight KN, Lac D, Leung AW, Lexa KW, Liau NPD, Becerra I, Malfavon M, McInnes J, Nguyen HN, Lozano EI, Pizzo ME, Roche E, Sacayon P, Calvert MEK, Daneman R, Dennis MS, Duque J, Gadkar K, Lewcock JW, Mahon CS, Meisner R, Solanoy H, Thorne RG, Watts RJ, Zuchero YJY, Kariolis MS.
Nat Commun. 2023 Aug 19. doi:10.1038/s41467-023-40681-4.

Epsilon toxin-producing Clostridium perfringens colonize the multiple sclerosis gut microbiome overcoming CNS immune privilege.
Ma Y, Sannino D, Linden JR, Haigh S, Zhao B, Grigg JB, Zumbo P, Dundar F, Butler D, Profaci CP, Telesford K, Winokur PN, Ruman KR, Gauthier SA, Fischetti VA, McClane BA, Uzal FA, Zexter L, Mazzucco M, Rudick R, Danko D, Balmuth E, Nealon N, Perumal J, Kaunzner U, Brito IL, Zhengming C, Xiang JZ, Betel D, Daneman R, Sonnenberg GF, Mason CE, Vartanian T.
J Clin Invest. 2023 May 1. doi:10.1172/JCI163239.

Editorial: Brain arteriovenousmalformations: cerebrovasculature behaving badly.
Shoemaker LD, Daneman R, Stoodley MA.
Front Hum Neurosci. 2023 June 7. doi:10.3389/fnhum.2023.1212184.

2022

Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging.
Bonney, SK, Sullivan LT, Cherry TJ, Daneman R, Shih AY.
J Cereb Blood Flow Metab. 2022 June. doi:10.1177/0271678X211068528.

Specification of CNS macrophage subsets occurs postnatally in defined niches.
Masuda T, Amann L, Monaco G, Sankowski R, Staszewski O, Krueger M, Del Gaudio F, He L, Paterson N, Nent E, Fernández-Klett F, Yamasaki A, Frosch M, Fliegauf M, Bosch LFP, Ulupinar H, Hagemeyer N, Schreiner D, Dorrier C, Tsuda M, Grothe C, Joutel A, Daneman R, Betsholtz C, Lendahl U, Knobeloch KP, Lämmermann T, Priller J, Kierdorf K, Prinz M.
Nature. 2022 Apr 20. doi: 10.1038/s41586-022-04596-2.

Engineered Wnt ligands enable blood-brain barrier repair in neurological disorders.
Martin M, Vermeiren S, Bostaille N, Eubelen M, Spitzer D, Vermeersch M, Profaci CP, Pozuelo E, Toussay X, Raman-Nair J, Tebabi P, America M, De Groote A, Sanderson LE, Cabochette P, Germano RFV, Torres D, Boutry S, de Kerchove d'Exaerde A, Bellefroid EJ, Phoenix TN, Devraj K, Lacoste B, Daneman R, Liebner S, Vanhollebeke B.
Science. 2022 Feb 18. doi: 10.1126/science.abm4459.

2021

Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging.
Bonney SK, Sullivan LT, Cherry TJ, Daneman R, Shih AY.
J Cereb Blood Flow Metab. 2021 Dec 20. doi: 10.1177/0271678X211068528.

Emerging roles for CNS fibroblasts in health, injury and disease.
Dorrier CE, Jones HE, Pintaric L, Siegenthaler JA, Daneman R.
Nat Rev Neurosci. 2021 Oct 20. doi:10.1038/s41583-021-00525-w.

Astrocytes propel neurovascular dysfunction during cerebral cavernous malformation lesion formation.
Lopez-Ramirez MA, Lai CC, Soliman SI, Hale P, Pham A, Estrada EJ, McCurdy S, Girard R, Verma R, Moore T, Lightle R, Hobson N, Shenkar R, Poulsen O, Haddad GG, Daneman R, Gongol B, Sun H, Lagarrigue F, Awad IA, Ginsberg MH.
J Clin Invest. 2021 July 1. doi: 10.1172/JCI139570.

CNS fibroblasts form a fibrotic scar in response to immune cell infiltration.
Dorrier CE, Aran D, Haenelt EA, Sheehy RN, Hoi KK, Pintaric L, Chen Y, Lizama CO, Cautivo KM, Weiner GA, Popko B, Fancy SP, Arnold TD, Daneman R.
Nat Neurosci. 2021 Feb 1. doi: 10.1038/s41593-020-00770-9.

2020

Neuronal activity regulates blood-brain barrier efflux transport through endothelial circadian genes.
Pulido RS, Munji RN, Chan TC, Quirk CR, Weiner GA, Weger BD, Rossi MJ, Elmsaouri S, Malfavon M, Deng A, Profaci CP, Blanchette M, Qian T, Foreman KL, Shusta EV, Gorman MR, Gachon F, Leutgeb S, Daneman R.
Neuron. 2020 Dec 9. doi: 10.1016/j.neuron.2020.09.002

Multidimensional proteome profiling of blood-brain barrier perturbation by Group B Streptococcus.
Campeau A, Mills RH, Blanchette M, Bajc K, Malfavon M, Munji RN, Deng L, Hancock B, Patras KA, Olson J, Nizet V, Daneman R, Doran K, Gonzalez DJ.
mSystems. 2020 Aug 25. doi: 10.1128/mSystems.00368-20.

Unexpected amount of blood-borne protein enters the young brain.
Munji RN, Daneman R.
Nature. 2020 Jul. doi:10.1038/d41586-020-01791-x.

2019

Delivering genes across the blood-brain barrier: Ly6A, a novel cellular receptor for AAV-PHP.B capsids.
Huang Q, Chan KY, Tobey IG, Chan YA, Poterba T, Boutros CL, Balazs AB, Daneman R, Bloom JM, Seed C, Deverman BE.
Plos One. 2019 Nov 14. doi:10.1371/journal.pone.0225206.

Profiling the mouse brain endothelial transcriptome in health and disease models reveals a core blood-brain barrier dysfunction module.
Munji RN*, Soung AL*, Weiner GA, Sohet F, Semple BD, Trivedi A, Gimlin K, Kotoda M, Korai M, Aydin S, Batugal A, Cabangcala AC, Schupp PG, Oldham MC, Hashimoto T, Noble-Haeusslein LJ, Daneman R.
Nat Neurosci. 2019 Oct 14. doi: 10.1038/s41593-019-0497-x

Cholinergic neural activity directs retinal layer-specific angiogenesis and blood retinal barrier formation.
Weiner GA*, Shah SH*, Angelopoulos CM, Bartakova AB, Pulido RS, Murphy A, Nudleman E, Daneman R*, Goldberg JL*.
Nat Commun. 2019 Jun 6;10(1):2477. doi: 10.1038/s41467-019-10219-8.

Human pluripotent stem cell-derived brain pericyte-like cells induce blood-brain barrier properties.
Stebbins MJ, Gastfriend BD, Canfield SG, Lee MS, Richards D, Faubion MG, Li WJ, Daneman R, Palecek SP, Shusta EV.
Sci Adv. 2019 Mar 13;5(3):eaau7375.

2018

In Memoriam: Ben Barres
Allen NJ, Daneman R.
J Cell Biol (2018) 217 (2): 435–438.

Activation of RARα, RARγ, or RXRα Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells.
Stebbins MJ, Lippmann ES, Faubion MG, Daneman R, Palecek SP, Shusta EV.
Biotechnol J. 2018 Feb;13(2).

2017

The amazing brain drain.
Blanchette M, Daneman R.
J Exp Med. 2017 Dec 4;214(12):3469-3470.

Brain barriers in health and disease.
Daneman R, Engelhardt B.
Neurobiol Dis. 2017 Nov;107:1-3.

Evolutionarily Conserved Roles for Blood-Brain Barrier Xenobiotic Transporters in Endogenous Steroid Partitioning and Behavior.
Hindle SJ*, Munji RN*, Dolghih E, Gaskins G, Orng S, Ishimoto H, Soung A, DeSalvo M, Kitamoto T, Keiser MJ, Jacobson MP, Daneman R*, Bainton RJ*.
Cell Rep. 2017 Oct 31;21(5):1304-1316.

Altered cargo proteins of human plasma endothelial cell-derived exosomes in atherosclerotic cerebrovascular disease.
Goetzl EJ, Schwartz JB, Mustapic M, Lobach IV, Daneman R, Abner EL, Jicha GA.
FASEB J. 2017 Aug;31(8):3689-3694.

A Basic ApoE-Based Peptide Mediator to Deliver Proteins across the Blood-Brain Barrier: Long-Term Efficacy, Toxicity, and Mechanism.
Meng Y, Wiseman JA, Nemtsova Y, Moore DF, Guevarra J, Reuhl K, Banks WA, Daneman R, Sleat DE, Lobel P.
Mol Ther. 2017 Jul 5;25(7):1531-1543.

2016

Finding NMO.
Daneman R.
Neurol Neuroimmunol Neuroinflamm. 2016 Dec 19;4(1):e313.

Peripheral and central neuronal ATF3 precedes CD4+ T-cell infiltration in EAE.
Frezel N, Sohet F, Daneman R, Basbaum AI, Braz JM.
Exp Neurol. 2016 Sep;283(Pt A):224-34

Oligodendrocyte precursors migrate along vasculature in the developing nervous system.
Tsai HH, Niu J, Munji R, Davalos D, Chang J, Zhang H, Tien AC, Kuo CJ, Chan JR, Daneman R, Fancy SP.
Science. 2016. Jan 22;351(6271):379-84

2015

Formation and maintenance of the BBB.
Blanchette M, Daneman R.
Mech Dev. 2015 Jul 24. pii: S0925-4773(15)30009-5

LSR/angulin-1 is a tricellular tight junction protein involved in blood-brain barrier formation.
Sohet F, Lin C, Munji RN, Lee SY, Ruderisch N, Soung A, Arnold TD, Derugin N, Vexler ZS, Yen FT, Daneman R.
J Cell Biol. 2015 Mar 16;208(6):703-11

The blood-brain barrier.
Daneman R, Prat A.
Cold Spring Harb Perspect Biol. 2015 Jan 5;7(1)

2014

Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier.
Nacer A, Movila A, Sohet F, Girgis NM, Gundra UM, Loke P, Daneman R, Frevert U.
PLoS Pathog. 2014 Dec 4;10(12):e1004528

Dissecting gene expression at the blood-brain barrier.
Huntley MA, Bien-Ly N, Daneman R, Watts RJ.
Front Neurosci. 2014 Nov 6;8:355

High endothelial venules through a transcriptomics lens.
Rosen SD, Daneman R.
Nat Immunol. 2014 Oct;15(10):906-8

An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex.
Zhang Y, Chen K, Sloan SA, Bennett ML, Scholze AR, O'Keeffe S, Phatnani HP, Guarnieri P, Caneda C, Ruderisch N, Deng S, Liddelow SA, Zhang C, Daneman R, Maniatis T, Barres BA, Wu JQ.
J Neurosci. 2014 Sep 3;34(36):11929-47

Oligodendrocyte-encoded HIF function couples postnatal myelination and white matter angiogenesis.
Yuen TJ, Silbereis JC, Griveau A, Chang SM, Daneman R, Fancy SP, Zahed H, Maltepe E, Rowitch DH.
Cell. 2014 Jul 17;158(2):383-96

Regulation of intrinsic axon growth ability at retinal ganglion cell growth cones.
Steketee MB, Oboudiyat C, Daneman R, Trakhtenberg E, Lamoureux P, Weinstein JE, Heidemann S, Barres BA, Goldberg JL.
Invest Ophthalmol Vis Sci. 2014 Jun 6;55(7):4369-77

Purification and culture of central nervous system pericytes.
Zhou L, Sohet F, Daneman R.
Cold Spring Harb Protoc. 2014 Jun 2;2014(6):581-3

Purification of pericytes from rodent optic nerve by immunopanning.
Zhou L, Sohet F, Daneman R.
Cold Spring Harb Protoc. 2014 Jun 2;2014(6):608-17

Purification of endothelial cells from rodent brain by immunopanning.
Zhou L, Sohet F, Daneman R.
Cold Spring Harb Protoc. 2014 Jan 1;2014(1):65-77

Purification and culture of central nervous system endothelial cells.
Zhou L, Sohet F, Daneman R.
Cold Spring Harb Protoc. 2014 Jan 1;2014(1):44-6

2013

Development, maintenance and disruption of the blood-brain barrier.
Obermeier B, Daneman R, Ransohoff RM.
Nat Med. 2013 Dec;19(12):1584-96

'Sealing off the CNS': cellular and molecular regulation of blood-brain barriergenesis.
Siegenthaler JA, Sohet F, Daneman R.
Curr Opin Neurobiol. 2013 Dec;23(6):1057-64

Foxc1 is required by pericytes during fetal brain angiogenesis.
Siegenthaler JA, Choe Y, Patterson KP, Hsieh I, Li D, Jaminet SC, Daneman R, Kume T, Huang EJ, Pleasure SJ.
Biol Open. 2013 May 20;2(7):647-59

Transporters of the blood-brain and blood-CSF interfaces in development and in the adult.
Saunders NR, Daneman R, Dziegielewska KM, Liddelow SA.
Mol Aspects Med. 2013 Apr-Jun;34(2-3):742-52

Genetic mouse models to study blood-brain barrier development and function.
Sohet F, Daneman R.
Fluids Barriers CNS. 2013 Jan 10;10(1):3

2012

The blood-brain barrier in health and disease.
Daneman R.
Ann Neurol. 2012 Nov;72(5):648-72

Blood-brain barrier permeability is increased after acute adult stroke but not neonatal stroke in the rat.
Fernández-López D, Faustino J, Daneman R, Zhou L, Lee SY, Derugin N, Wendland MF, Vexler ZS.
J Neurosci. 2012 Jul 11;32(28):9588-600

2011

Development of a method for the purification and culture of rodent astrocytes.
Foo LC, Allen NJ, Bushong EA, Ventura PB, Chung WS, Zhou L, Cahoy JD, Daneman R, Zong H, Ellisman MH, Barres BA.
Neuron. 2011 Sep 8;71(5):799-811

Tyrosine kinase inhibitors ameliorate autoimmune encephalomyelitis in a mouse model of multiple sclerosis.
Crespo O, Kang SC, Daneman R, Lindstrom TM, Ho PP, Sobel RA, Steinman L, Robinson WH.
J Clin Immunol. 2011 Dec;31(6):1010-20

2010

The mouse blood-brain barrier transcriptome: a new resource for understanding the development and function of brain endothelial cells.
Daneman R, Zhou L, Agalliu D, Cahoy JD, Kaushal A, Barres BA.
PLoS One. 2010 Oct 29;5(10):e13741

Pericytes are required for blood-brain barrier integrity during embryogenesis.
Daneman R, Zhou L, Kebede AA, Barres BA.
Nature. 2010 Nov 25;468(7323):562-6

2009

The gut immune barrier and the blood-brain barrier: are they so different?
Daneman R, Rescigno M.
Immunity. 2009 Nov 20;31(5):722-35

Wnt/beta-catenin signaling is required for CNS, but not non-CNS, angiogenesis.
Daneman R, Agalliu D, Zhou L, Kuhnert F, Kuo CJ, Barres BA.
Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):641-6

2008

A novel purification method for CNS projection neurons leads to the identification of brain vascular cells as a source of trophic support for corticospinal motor neurons.
Dugas JC, Mandemakers W, Rogers M, Ibrahim A, Daneman R, Barres BA.
J Neurosci. 2008 Aug 13;28(33):8294-305

2005

The blood-brain barrier--lessons from moody flies.
Daneman R, Barres BA.
Cell. 2005 Oct 7;123(1):9-12